luciferase reporter plasmids prl-sv40 (Promega)
90
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Promega
luciferase reporter plasmids prl-sv40
Luciferase Reporter Plasmids Prl Sv40, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-sv40+plasmids/pgl3+basic/pmc10266032__pnas__2219419120__sapp-162-24-35
Average 90 stars, based on 1 article reviews
Luciferase Reporter Plasmids Prl Sv40, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-sv40+plasmids/pgl3+basic/pmc10266032__pnas__2219419120__sapp-162-24-35
Average 90 stars, based on 1 article reviews
luciferase reporter plasmids prl-sv40 - by Bioz Stars,
2026-09
90/100 stars
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Activity Assay:Article Title: TAp63α Is Involved in Tobacco Smoke-Induced Lung Cancer EMT and the Anti-cancer Activity of Curcumin via miR-19 Transcriptional Suppression Article Snippet: Luciferase reporter gene plasmids of the wt-miR-17-92 promoter and mut-miR-17-92 promoter were obtained from GENEray (Shanghai, China). Article Title: MiR-30a-5p isoform -1|1 promotes the progression of gastric cancer by inhibiting TMEM66 and reducing intratumoral cytotoxic T cells. Article Snippet: Gastric cancer is histologically classified into the intestinal subtype, which forms tubular structures, and the aggressive diffuse subtype, characterized by rapid invasion and poor prognosis.. The variety and quantity of miRNA isoforms between different histological subtypes of gastric cancer were unknown.. Through systematic filtering, we found that more diverse miR-30a-5p isoforms was present in the diffuse subtype of gastric cancer, and was associated with patients’ worse survival independent of tumor stage based on the TCGA miRNA-seq data. Article Title: Skatole regulates intestinal epithelial cellular functions through activating aryl hydrocarbon receptors and p38. Article Snippet: Intestinal bacteria produce skatole (3-methylindole) from tryptophan in dietary proteins and ingesting large quantities of animal protein is associated with increased fecal skatole concentrations.. Although possibly associated with disrupted intestinal homeostasis, the influence of skatole on intestinal epithelial cellular function has not been characterized in detail.. The present study aimed to determine whether skatole induces intestinal epithelial cell (IEC) dysfunction. Article Title: Hexaraphane Affects the Activation of Hepatic PPARα Signaling: Impact on Plasma Triglyceride Levels and Hepatic Senescence with Aging. Article Snippet: PPRE transcriptional activity was measured in HepG2 cells transfected with PPRE X3-TK-luc (Addgene, Cambridge, MA, USA) and Article Title: Deregulated GATA6 modulates stem cell-like properties and metabolic phenotype in hepatocellular carcinoma. Article Snippet: Accumulating evidence illustrates the significance of cell plasticity in the molecular biology of liver cancer.. Reprogramming of mature parenchymal cells to a less differentiated state by key molecular targets contributes to the pathogenesis of hepatocellular carcinoma (HCC).. Hereby we investigated the role of GATA6, a transcription factor implicated in hepatocyte lineage specification, in HCC. Article Title: Interleukin-17A mediates tobacco smoke-induced lung cancer epithelial-mesenchymal transition through transcriptional regulation of ΔNp63α on miR-19. Article Snippet: Interleukin-17A (IL-17A) is an essential inflammatory cytokine in the progress of carcinogenesis.. Tobacco smoke (TS) is a major risk factor of lung cancer that inf luences epi thel ial mesenchymal transition (EMT) process.. However, the potential mechanism by which IL-17A mediates the progression of lung cancer in TS-induced EMT remains elusive. Article Title: Oxidative stress-mediated tetrabromobisphenol A disrupts mitochondrial function in HepG 2 cells and activates ferroptosis signalling to induce apoptosis. Article Snippet: Tetrabromobisphenol A (TBBPA), a brominated flame retardant extensively used in consumer electronics, has been classified as a persistent environmental contaminant.. While TBBPA-induced mitochondrial dysfunction is implicated in apoptosis, the precise oxidative stress-mediated mechanisms remain incompletely characterized.. In this study, using human liver cancer cells (HepG2) as an in vitro model, we systematically investigated TBBPA’s mitochondrial toxicity and associated cell death pathways. Transfection:Article Title: TAp63α Is Involved in Tobacco Smoke-Induced Lung Cancer EMT and the Anti-cancer Activity of Curcumin via miR-19 Transcriptional Suppression Article Snippet: Luciferase reporter gene plasmids of the wt-miR-17-92 promoter and mut-miR-17-92 promoter were obtained from GENEray (Shanghai, China). Article Title: MiR-30a-5p isoform -1|1 promotes the progression of gastric cancer by inhibiting TMEM66 and reducing intratumoral cytotoxic T cells. Article Snippet: Gastric cancer is histologically classified into the intestinal subtype, which forms tubular structures, and the aggressive diffuse subtype, characterized by rapid invasion and poor prognosis.. The variety and quantity of miRNA isoforms between different histological subtypes of gastric cancer were unknown.. Through systematic filtering, we found that more diverse miR-30a-5p isoforms was present in the diffuse subtype of gastric cancer, and was associated with patients’ worse survival independent of tumor stage based on the TCGA miRNA-seq data. Article Title: Skatole regulates intestinal epithelial cellular functions through activating aryl hydrocarbon receptors and p38. Article Snippet: Intestinal bacteria produce skatole (3-methylindole) from tryptophan in dietary proteins and ingesting large quantities of animal protein is associated with increased fecal skatole concentrations.. Although possibly associated with disrupted intestinal homeostasis, the influence of skatole on intestinal epithelial cellular function has not been characterized in detail.. The present study aimed to determine whether skatole induces intestinal epithelial cell (IEC) dysfunction. Article Title: Hexaraphane Affects the Activation of Hepatic PPARα Signaling: Impact on Plasma Triglyceride Levels and Hepatic Senescence with Aging. Article Snippet: PPRE transcriptional activity was measured in HepG2 cells transfected with PPRE X3-TK-luc (Addgene, Cambridge, MA, USA) and Article Title: Deregulated GATA6 modulates stem cell-like properties and metabolic phenotype in hepatocellular carcinoma. Article Snippet: Accumulating evidence illustrates the significance of cell plasticity in the molecular biology of liver cancer.. Reprogramming of mature parenchymal cells to a less differentiated state by key molecular targets contributes to the pathogenesis of hepatocellular carcinoma (HCC).. Hereby we investigated the role of GATA6, a transcription factor implicated in hepatocyte lineage specification, in HCC. Article Title: Interleukin-17A mediates tobacco smoke-induced lung cancer epithelial-mesenchymal transition through transcriptional regulation of ΔNp63α on miR-19. Article Snippet: Interleukin-17A (IL-17A) is an essential inflammatory cytokine in the progress of carcinogenesis.. Tobacco smoke (TS) is a major risk factor of lung cancer that inf luences epi thel ial mesenchymal transition (EMT) process.. However, the potential mechanism by which IL-17A mediates the progression of lung cancer in TS-induced EMT remains elusive. Article Title: Oxidative stress-mediated tetrabromobisphenol A disrupts mitochondrial function in HepG 2 cells and activates ferroptosis signalling to induce apoptosis. Article Snippet: Tetrabromobisphenol A (TBBPA), a brominated flame retardant extensively used in consumer electronics, has been classified as a persistent environmental contaminant.. While TBBPA-induced mitochondrial dysfunction is implicated in apoptosis, the precise oxidative stress-mediated mechanisms remain incompletely characterized.. In this study, using human liver cancer cells (HepG2) as an in vitro model, we systematically investigated TBBPA’s mitochondrial toxicity and associated cell death pathways. |
![A ChIP peak locations within the human EIF4EBP1 promoter, exon 1 and part of intron 1 (hg38; Chr8: 38,030,342 - 38,031,906) from ChIP-sequencing data for MYC (Encode consortium, Encyclopedia of DNA Elements at UCSC [ , ]) and an illustration of the <t>luciferase</t> reporter construct containing the EIF4EBP1 promoter, exon 1 and part of intron 1 (−192; +1372) coupled to Firefly luciferase, with the indicated binding sites of the transcription factor MYC. The three E boxes present in the promoter, and corresponding introduced mutations, are indicated. B , C HEK293-T cells were transfected with the (−192; +1372) EIF4EBP1 promoter reporter construct, together with 25 ng, 50 ng and 100 ng MYC ( B ) or the (−192; +1372) EIF4EBP1 promoter reporter constructs containing a mutation of each of the E boxes (as indicated in A ), together with 25 ng MYC ( C ). For ( B ) and ( C ), a <t>Renilla</t> Luciferase vector was used as an internal control and luciferase activities were detected using the Dual-Luciferase Reporter Assay. Firefly luciferase activity was normalized to Renilla luciferase activity and the ratio was normalized to the corresponding 0 ng ( B ) or control ( C ) condition. Data represent the mean of four ( B ) or three ( C ) independent replicates ± standard deviation (SD). Significance was calculated using an unpaired and two-tailed parametric t test (* p < 0.05, **** p < 0.0001). A representative immunoblot analyzing expression of MYC is presented in ( B ). D , E Med8A ( D ) and HD-MB03 ( E ) MB cells were transiently transfected with negative control siRNAs (siCtrl), or two different siRNAs each targeting MYC (siMYC#1 and siMYC#2). Cells were re-transfected after 96 h with their corresponding siRNA and incubated for a total of 168 h. MRNA was harvested to determine the expression levels of EIF4EBP1 and MYC by qRT-PCR. Data obtained by qRT-PCR represent the mean of three independent replicates ± SD and the fold change in expression was normalized to the negative control (siCtrl). Significance was calculated using an unpaired and two-tailed parametric t test (* p < 0.05, ** p < 0.01, **** p < 0.0001). F Med8A MB cells were transiently transfected with negative control (siCtrl) or a pool of four different siRNAs (see Table ) targeting MYC (siMYC). Cells were incubated 72 h and protein was harvested for immunoblotting using the indicated antibodies. G , H Control and MYC overexpressing (MYC OE) ONS76 ( G ) or UW228.3 ( H ) cells were lysed. Levels of EIF4EBP1 mRNA were determined by qRT-PCR. Levels of 4EBP1 and MYC proteins were determined by immunoblots using the indicated antibodies. Data obtained by qRT-PCR represent the mean of three independent replicates ± SD and the fold change in expression was normalized to the control. Significance was calculated using an unpaired and two-tailed parametric t test (*** p < 0.001, **** p < 0.0001).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7489/pmc12267489/pmc12267489__41420_2025_2601_Fig4_HTML.jpg)